The mapping population used in this study (A05141) consisted of 162 F1 progeny derived from a cross between ‘Rio Grande Russet’ (female parent) and ‘Premier Russet’ (male parent). ‘Premier Russet’ is a dual purpose (processing and fresh market) potato variety with significant resistance to the accumulation of reducing sugars after long-term storage at low temperatures . Rio Grande Russet is a high yielding fresh market potato variety . A05141 was developed at the USDA-ARS Small Grains and Potato Germplasm Research Unit (Aberdeen, ID) and was one of the mapping populations phenotyped and genotyped by the Solanaceae Coordinated Agricultural Project (SolCAP) .
Profession experiments was indeed conducted within USDA-ARS, College out-of Idaho Lookup and Expansion Cardiovascular system (Aberdeen, ID) and you can New york County School Tidewater Search Route (Plymouth, NC) inside the summers from 2010 and you can 2011. The progeny and you will mothers had been analyzed inside the a great randomized complete block build which have a couple of replications away from 10-slope plots of land at each location. Vine readiness is actually visually obtained to the a-1–9 scale on 95 (95DAP) and you can 120 (120DAP) days shortly after planting, which have step 1 = early, 100% vines inside the a land dead, whenever you are 9 = most later, complete bloom that have buds obvious. Plant growth practice is actually registered early in blooming and you may expressed since the 3 = erect (> 45° which have surface), 5 = semi-erect (30–45° that have ground), and you may seven = dispersed (More file ten: Desk S5). Verticillium wilt and you may early blight (EB) (Altenaria tenuis) sickness was basically ranked with the a great 0–nine level. To possess EB, 0 suggests zero condition and 9 > 90% of the leaf affected area from inside the a story. Having VW, the dimensions try a combined score to have leaf area/stalk flagging as a result of the condition (Even more file ten: Dining table S5). Plots of land just weren’t inoculated, however, Aberdeen industries enjoys highest inoculum of Verticillium in the crushed, collected more years. Early blight is seen annual at ID and you may NC cities and for that it demonstration the condition is permitted to produce obviously, without fungicides applied for its handle.
Tuber products had been addressed with Isopropyl Chlorpropham (N-(3-chlorophenyl) carbamate) at a rate out-of 22 ppm to cease sprouting and held at 42 °F and you can 95% cousin dampness to have 3 months.
Glucose density into the solutions contained in this demo have been computed of an effective ten-tuber try utilising the style of Sowokinos ainsi que al. which have changes. Tubers was basically reduce having fun with an enthusiastic Kut Footwear Stringer French fry cutter. 2 hundred grams away from tuber cells accumulated throughout the center out of new ten tubers was indeed macerated during the an enthusiastic Acme Juicerator (Acme Equipment, Springtime Slope, FL). Throughout the control, tuber muscle is sparkling that have 150 mL from sodium-phosphate barrier (0.05 M, pH 7.5) to own a final homogenate quantity of 275 mL. Glucose concentrations were determined having fun with a YSI model 2700 Analyzer (Purple Springs Appliance Co., Inc., Purple Springs, OH) and you will indicated towards the a percent fresh weight foundation.
Bud-stop and you can Stalk-end fry colour
A remove away from tissue (step three.0 cm ? 0.8 cm) taken from all the ten tubers included in the brand new glucose extraction techniques was applied to own fry colour determination (ten pieces for every imitate). Pieces was indeed deep-fried inside the canola oil in the 191 °C to own step three.5 min. Fry color try calculated inside step escort Bellevue three min using a product 577 Photovolt Meditation Meter (design 577, Photovolt Tools Inc., Minneapolis, MN). An eco-friendly filter was utilized additionally the instrument try calibrated playing with a black colored-cavity fundamental just like the 0.0% reflectance and you can a white plaque (Pet. No. 26-5Cat. Zero. 26–570-08) given that 99.9% reflectance. Specifications was adopted the brand new concludes of each remove. The lower brand new reflectance measurement, the fresh dark the fresh fry colour and you can vice versa.